TThis comprehensive book with 338 references discusses on the development of analytical systems based on flow analysis techniques with the chemiluminescence (CL) detections. The application of different flow-based methodologies, including flow injection analysis (FIA), sequential injection analysis (SIA), multicommutation flow injection analysis (MCFIA) and multisyringe flow injection analysis (MSFIA) combined with CL reactions, has been discussed. Several CL systems, including those based on luminol, potassium permanganate, cerium(IV) sulphate and ruthenium(II) complexes, among others, are critically presented. The reported information in the papers published over the last 4 years have been classified in instrumental developments and application areas including food analysis, environmental analysis, pharmaceutical analysis, detection and quantification of DNA and other bio-related species, antioxidant assessment and miscellaneous. Following the review of the analytical application concepts, the mechanisms of the CL reactions are also briefly discussed.