Transgenic crop is at present a demand of time. Through breeding and agricultural techniques we have achieved the highest production possible. But demand of food is still increasing. So to narrow the gap of demand and production let us adopt GM crops. But it is not easy to develop such crops. Here is a story of transgenic tobacco developed following all the standard procedure to give a clear picture of the science behind developing a transgenic. During this research unique problems aroused which are documented here in this book to acquaint you with the unexpected and conquer those.