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Genes Analysis Expressed under Phosphorus Deficiency by promoter GUS


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  • Product Description

Root specific expression of the GUS reporter gene was observed when transformed plants were grown fully inside solid media both under ?P and +P and on MS media containing kanamycin. However, there was no GUS activity when plants were grown on the surface of the solid media and hydroponically under ?P and +P conditions. Morphological root assessment for the knockout plants has shown no difference between the knockout genotypes and the wild type. Thus, contrary to previous result, in this study other stress than phosphorus deficiency factors were assumed to be contributing for the expression of the GUS reporter gene in all the constructs. Oxygen stress, mechanical impedance and stress due to the selective marker during seedling growth were thought to be possible reasons. However, since the effect of growing conditions was not evaluated before, this investigation needs further verification. Additionally, quantifying the GUS reporter gene activity in the transformant plants grown under - P and +P conditions by real time PCR helps to explore the real function of these genes.

Product Specifications
SKU :COC99408
Number of Pages104
Publishing Year2011-06-12T00:00:00.000
Edition1 st
Book TypeGenetics (non-medical)
Country of ManufactureIndia
Product BrandNot defined
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-08-18 00:00:00