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Genetic Transformation of Potato

 

Marketed By :  LAP LAMBERT Academic Publishing   Sold By :  Kamal Books International  
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  • Product Description
 

Iron deficiency is the most wide spread nutritional problem in the world. For increasing the iron contents in four potato cultivars, in vitro leaf derived calli were transformed with Agrobacterium tumefaciens strain LBA4404 bearing Ti based binary pCAMBIA2301 plasmid containing selectable marker neomycin phosphotransferase (nptII) and ferritin gene. Maximum kanamycin resistant and ferriitn positive calli with the highest transformation frequency were obtained from Japanese Red by using 50 mg/l kanamycin, 50 mg/l cefotaxime, the bacterial suspension (OD=1.00 at 600 nm), 70 seconds incubation period, 24 h co-cultivation period in the presence of acetosyringone (25 mg/l). Putative transgenic plants were regenerated from the infected calli in medium containing MS + 3.0 mg/l BA + 0.1 mg/l NAA + 50 mg/l kanamycin + 50 mg/l cefotaxime. Kanamycin resistant plants were analysed with PCR and RT-PCR for the detection of ferritin gene and its transcript which reveal that ferritin genes were integrated into the plant genome.

Product Specifications
SKU :COC87424
AuthorUmme Qulsum,Monzur Hossain and Rafiul Islam
LanguageEnglish
BindingPaperback
Number of Pages120
Publishing Year2013-02-09T00:00:00.000
ISBN9783659326462
Edition1 st
Book TypeBotany & plant sciences
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-10-08 00:00:00