Drought is the most common adverse environmental condition that can seriously reduce crop productivity. Increasing crop resistance to drought stress would be the most economical approach to improve agricultural productivity and to reduce agricultural use of fresh water resources. As a result, understanding the drought tolerance and breeding for drought resistant crop plants has been the major goal of plant breeders. Sorghum is well-known for its capacity to tolerate conditions of limited moisture and to produce during periods of extended drought. Twenty sorghum (Sorghum bicolor L. Moench) genotypes with known drought tolerance traits were used in this study. The present study will be conducted to evaluate the genetic variability for drought tolerance in sorghum genotypes at molecular level.