This study was to determine the prevalence of Ureaplasma urealiticum and Mycoplasma hominis infection in women attending the University of Yaounde teaching Hospital (UYTH). Genital mycoplasma was found in 65% patients and distributed as 41% for Ureaplasma urealiticum and 4% for Mycoplasma hominis whilst there was co infection in 20% women. There was no relationship between genital Mycoplasmas and age group. In this study 57% had other organisms which included C. albicans 19%, G. vaginalis 35% and T. vaginalis 3%. Amongst the 65 women with genital mycoplasma, the highest coinfection was with G. vaginalis (33.8%). Antibiotic sensitivity showed Pristinamycine (PT) as the most sensitive antibiotic with 92% sensitivity and Trimethoprim-sulfamethoxazole (SXT) as the most resistant with a sensitivity of 8%. There is a relationship between genital Mycoplasma infection and C. albicans, G. vaginalis and T vaginalis. Abstinence and the use of condoms should be encouraged. Infected women should be treated together with their partners.