One of the "hottest" areas of current research involves a family of highly conserved stress proteins known as heat shock proteins (HSPs). These proteins are ubiquitous, occurring in all organisms from bacteria, plants and yeast to humans. Abiotic stimuli, such as heat, cold, water deficit, heavy metals, ozone, UV radiation and ?-irradiation effects the synthesis and accumulation of HSPs. Chloroplast Small Heat Shock Proteins are nuclear-encoded plastid localized HSPs, synthesized in response to heat stress in plants. Recently, it is demonstrated that Cp-sHSPs play an important role to protect photosynthetic electron transport during heat stress. We have studied the gene identification and expression of chloroplast specific small heat shock proteins under heat stress in Chenopodium album. The difference in expression pattern was seen at heat stress of 35°C to 40°C which gives the evidence of transcriptional or post transcriptional regulation. No transcript was seen at heat stress of 45°C, indicating the degradation of Cp-sHSP transcript at high temperature. Similarly, lack of transcript in control samples are shown that this gene is not transcribed at normal growth temperature.