Sugarcane is an important cash crop for many tropical and subtropical countries which account for approximately 75% of global sugar production. It is one of the most important cash crops of Pakistan with annual production of 63.92 million tons of cane cultivated on 1241 thousand hectares of land. The worldwide rank of Pakistan is fifth on the basis of area on which sugarcane is cultivated while it is ranked fifteenth on the basis of yield. Conventional breeding methods pose some serious threats for its safe growth and account for heavy losses of the crop every year. The use of plant tissue culture techniques for the propagation of sugarcane has overcome many problems associated with the traditional breeding methods by ensuring the disease free multiplication of the plants and reducing the time period required for the multiplication.