Hydrogel is a network of polymer chains, sometimes found as a colloidal gel form in which water is the dispersion medium. Present study evaluated the cell viability and cytotoxicity of the hydrogel, thereby providing preliminary information for further studies on systemic toxicities. In order to perform a convincing evaluation of the cytotoxicity of the hydrogel, various experiment concerning different aspects of cell damage were used which is described in detail in this book. It may open new regimes for the early detection and treatment of a number of diseases ranging from infectious disease to cancer. Studies on toxicity and pharmacokinetics of these hydrogel in in-vivo system could be further assessed.