This research has highlighted the impact of organizational culture on role based performance of the employees. It has also heightened the importance of the strong organizational culture that affect the employees role base and general performance. In this research there were 291 male which were 72.8% of the whole sample and 109 female that were 27.3% of the whole sample. Questionnaire was used to collect data of middle level managers of banking sector via non probability technique that is convenient sampling. Mann-whitney test showed that while considering grouping variable gender and status impact of organizational culture was equal in both male and female. There was some variation in male and female while considering nature of the family. While Regression Analysis showed a strong positive relationship between organization culture and its impact on role based performance.