Cancer is a second leading cause of death in the world. It has been a lot of research being done on cancer therapeutics but the deadliness of this disease has not been cured. This book insights into development of new avenue of synthetic compounds and their assessment in terms of effectiveness against major kinds of cancers i.e. lymphoma, breast cancer, and layrnx cancer along with safety study on kidney cells in vitro. In addition, it also describes number of assays available for screening. This can be useful to the students working on cancer research in chemistry, biochemistry as well as in vitro cytotoxicity screening departments. Also this can be a guide to usher lights to the budding researchers on their pathways towards synthetic therapies for different types of cancer and related diseases.