Egypt has the largest buffalo population in the Middle East and Africa (3.717 million); they act as a major source of red meat as well as the major producer of milk with a high efficiency of converting poor quality roughage into meat and milk. In addition, buffaloes are inherently resistant to many climatic stresses. However buffaloes are reported to have low reproductive performance with inherent reproductive problems of weak/silent estrus signs, seasonal anestrous. So, attention has been towards to biotechnologies as a way for overcome these problems and moreover to augment the reproduction potential of these animals. The present book is provide a detailed research on in vitro production of buffalo embryos, studying extensively the changes occurred in the oocyte morphology and morphometry during oocyte maturation, the stages of embryonic development after fertilization and the developmental kinetics of the in vitro produced embryos. This book” In vitro buffalo embryonic development” is considered a laboratory guide for most sufficient procedures for in vitro technique.