In vitro maturation (IVM) and fertilization (IVF) is the basic requirement of in vitro production (IVP) of embryos. The cumulus cells (CCs) surrounding the oocyte plays a key role of cytoplasmic maturation. It supplies nutrients, energy substrates and/or messenger molecules for the development of oocyte. Oocyte collection techniques have a significant role in IVM. Maturation media also plays a vital role on developmental potential of oocytes in IVP procedure. To establish a well-defined medium different serum have been extensively supplemented to the basic medium. Follicular fluid after necessary treatments could also be used as an economic supplement in the culture media (TCM-199).