Ginger (Zingiber officinale Rosc.) is the second most widely cultivated spice in Ethiopia, next to chilies. Recently, there has been huge demand for clean planting material of improved ginger cultivars, though it is difficult to meet the demand of planting materials using the conventional propagation techniques due to production inefficiency and disease transmission. Therefore, the present study was carried out with the objective of assessing the potential of axillary buds and shoot tips as explant sources and determination of suitable growth regulators for in vitro propagation of two ginger cultivars. A highly significant difference (p>0.0001) was observed between explant sources and among growth regulators for shoot multiplication and rooting.