Call Us 080-41656200 (Mon-Sat: 10AM-8PM)
Free Shipping above Rs. 1499
Cash On Delivery*

In vitro Regeneration and Genetic Transformation of Ricinus communis

 

Marketed By :  LAP LAMBERT Academic Publishing   Sold By :   
Delivery in :  2-7 Business Days

 

Check Your Delivery Options

 
Rs. 4,396

Availability: In stock

 
  • Product Description
 

An efficient in vitro plant regeneration and Agrobacterium tumefaciens mediated genetic transformation protocol was described for castor (Ricinus communis L.) using meristem as explants. Shoot apex containing apical meristems were excised from 5-7 days old in vitro grown seedlings and cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins singly or in combination. Kinetin (0.50 mg/l) in combination with BAP (0.25 mg/l) produced maximum number of shoot (10.33) and shoot length (5.20 cm). For root induction, in vitro produced shoots were transferred to rooting media containing ½ MS basal media with NAA. In Agrobacterium tumefaciens mediated genetic transformation, Agrobacterium tumefaciens strain containing construct pBIN1F harboring nptII and cry1F gene was used. The integration of the gene was confirmed by using PCR. The transformation experiment was performed by optimizing age of the seedlings and co-cultivation duration with Agrobacterium. When the effect of the age of the seedlings and co-cultivation duration evaluated fifteen days old seedlings and co-cultivated for three days yielded the frequency of transformation 2%.

Product Specifications
SKU :COC44132
AuthorRohan V. Kansara,Sanjay Jha and Suman Kumar Jha
LanguageEnglish
BindingPaperback
Number of Pages128
Publishing Year2012-01-23T00:00:00.000
ISBN978-3847374268
Edition1 st
Book TypeAgriculture & farming
Country of ManufactureIndia
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-06-08 00:00:00
0 Review(s)