Several hundreds of plant genera are used medicinally and plants are vital sources for potent and powerful drugs. The present study was conducted to screen the pharmacological activity of the ethanol and acetone extract of Phyllanthus amarus, Acalypha and indica Datura metel for its antimicrobial activity against selected pathogen. The bacterial Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa and Bacillus subtilis. The fungal Aspergillus niger and Penicilliumsp. The collected leaf samples were powdered and the bioactive compounds were extracted by using ethanol and acetone in a Soxhelet extractor. The antimicrobial activity was determined by using Disc diffusion method. Ethanol and acetone extracts with different concentrations (100mg/ml, 200mg/ml and 300mg/ml) were mixed with 1 ml of DMSO. The inhibitory effect of ethanol extract was relatively high when compared to acetone extract. The study of antimicrobial activity of herbal plant extract of Datura metel, Acalypha indica and Phyllanthus amarus showed that the ethanol extract shows promising antimicrobial activity against bacterial and fungal human pathogens when compared to acetone extact.