1. Introduction ? Types of reactive oxygen species (ROS) ? Sources ? Structure ? Mechanism of ROS generation ? p53,oxidative stress and ROS. 2. Oxidative stress and free radical damage ? Oxidative lipid damage ? Oxidative DNA damage ? Oxidative protein damage ? Role of ROS in cell signaling ? Aberrant regulation of redox-sensitive signal pathway ? Molecular mechanism of age-related diseases. 3. Antioxidant defense ? Antioxidant therapy ? Oxidative modification of protein and possible biomarkers. 4. Specific pathways transduced by ROS and ROS-induced apoptosis will provide opportunities to impact both life span, Age related-diseases and design the chemotherapeutics for humans.