Nano Biotechnology plays a vital role in the field of Infection especially virus. In this edition,enormous material has been included wherever felt necessary to update the book. To provide new information about Iron Nano particles interact with Influenza virus, two chapters are elaborately discussed in this context. I extend my heartfelt thanks to Dr.S.Kannan, (Assistant Professor in Zoology)for his valuable suggestion. Readers perception in the form of constructive suggestions and valuable comments for improvement of the book is highly appreciated.