This study was designed to investigate the possibility of infection of camel rearing communities with T. evansi in the study areas in the Sudan using classical and new diagnostic techniques of molecular biology include: Polymerase Chain Reaction (PCR) and Loop mediated-isothermal Amplification (LAMP). Four Sudanese States were chosen as study areas for this study on the light of the density of camel population. Subjects of the study were camels and humans in close contact with camels; those included: camel rearing communities, Veterinarians and slaughter houses labours. A total of 1186 blood samples were collected from camels and 336 blood samples from humans. All samples were screened for T. evansi infection using parasitological, serological and molecular tests. None of the human samples were positive parasitologically using Giemsa stained slides. Whereas 23 camel samples were positive (1.94%). Combination of parasitological, serological and advanced molecular diagnosis methods could give an optimum satisfied result to estimate the prevalence of trypanosomiasis. This will help to design proper integrated control strategies to combat the disease in the endemic areas.