Bacteria is the first form of life on Earth. All living creatures were born free of microorganisms but subsequently acquire them and evolve in their presence, integrations and interactions. Probiotic bacteria (Probiotics) have been gaining increasing interest in recent research. Probiotics are universally used in food industry, dietary supplements, to provide immunity and to prevent many diseases. This manuscript presents some details about the Sudanese traditional dairy products, uses, microbiota present & research conducted. Conventional methods of bacterial identification were used and molecular techniques were also included. Several tests for characterization of Lactic Acid Bacteria as probitics were described. This study is useful guide to undergraduate & post-graduate students. It helps the Sudanese and international researchers to be aware of the probiotics as new emerge bacteriotherapeutic agents.