Present research work embodies the different methods of Jatropha propagation by using seed germination, stem cutting and tissue culture methods. The extracts of leaf, stem and roots of Jatropha curcas L. were screened in order to study their effects on plant pathogenic fungi, plant pathogenic bacteria and human pathogenic bacteria. Interestingly antibacterial and antifungal nature of plant extracts have been noted. Regarding biochemical analysis of Jatropha species, the studies have made in the part second of the research work and the results are found to be useful in order to record the presence of certain bioactive chemicals in the plants. In order to achieve a mutant ideal plant the studies were carried out by using different mutagenic agents where results showed that certain mutagens are found to be helpful to achieve early flowering and fruiting in Jatropha curcas L.