Keratinases from microorganisms have attracted a great deal of attention due to their multitude of industrial applications. The most promising application of Keratinases/ Keratinolytic microorganisms is the production of nutritious, cost-effective, environmentally benign feather meal for poultry. In the present investigation an Isolate RS1 was emerged as a best candidate to be utilized in waste management of keratin wastes, leather, detergent and X ray film recycling. Thus, the strain and its enzyme can be utilized in the bioremediation of keratinolytic wastes for the production of fertilizer, poultry feed and also to solve the “Solid waste problem” created by these wastes. Complete dehairing of the skin and other important applications were also achieved with enzyme in this present study.