Kisspeptin is a key player and regulator of reproduction and exerts profound effects on male reproductive processes. Degenerative effects of critical regulators of reproduction, the kisspeptin peptides, on cellular aspects of sexually immature male gonads are known but similar information on accessory sex glands remain elusive. This book, therefore, assess the effects of kisspeptin treatment by evaluating the cellular architecture at the light and electron microscopic level and by determining the height of epithelium in seminal vesicles. In addition DNA damage was analyzed by DNA ladder assay and DNA fragmentation assay. Kisspeptin-10 exerted a pronounced inhibitory effect on seminal vesicles. It reduced the height of epithelium and weight of seminal vesicles and caused considerable DNA damage. Light microscopic examination evidenced dilatation of the lumen and lack of intricate epithelial folds. Ultrastructural changes included dilatation of the endoplasmic reticulum and Golgi cisterns, a reduction of secretory vesicles and the appearance of autophagic vacuoles. This is the preliminary study of the effect of kisspeptin on seminal vesicles.