Amphibians watched the dinosaurs come and go, but today almost half of them are threatened with extinction. Amphibian species diversity is highest in the tropics. Hibernation is usually thought of as a resting condition in which an animal exists in a state of complete or partial torpidity. This is associated with low temperature. During this time animal''s dramatically slowing heart and breathing rates, lowering body temperature, going for long periods without eating. Biochemical and physiological parameters played very crucial role during hibernation. Antioxidant activity, enzyme expression pattern, RT-PCR analysis help to understand the hibernation biology.