This manual is more specific to medical students’ especially first professional of Biochemistry. This book contains a huge variety of experiments related to carbohydrate, lipid and protein identification. Other contents of the book are, making of solutions (percentage, molar, normal and dilution) and buffer preparation of required pH by using Henderson Hasselbach equation. For medical and biochemistry students, this book also contains critical VIVA and OSPE questions with well defined answers. This book is a complete package for first year MBBS biochemistry class.