Cryopreservation is a science that can be learnt only through practice. This Manual is an attempt to educate the learners and readers of this topic to acquire guidance and directions to do cryopreservation with fewer chances for failure. Aspects like fish spermatozoa evaluation and protocol for freezing are dealt in elaborate in order to explicitly educate the researchers, students and scientists. Deviating from the normal frame of text book and providing theoretical explanation, attempts have been made to guide the readers in practical aspects of cryopreservation and artificial fertilization of fish gametes. This manual is an outcome of practical research done in the collection, evaluation and cryopreservation of milt. The problems that are normally encountered in such work are also hinted at appropriate places. The suggested readings at the end of the manual can be additional information to the readers. Although every effort is made to provide the best in class manual to help all working in this line, the readers are encouraged to refer further research publications and expand their knowledge.