The water samples were collected for the isolation of strains of alga Spirulina platensis from three different locations and the strain Spirulina platensis was isolated and it was, designated as ANS - 1 strain. The characteristics of Spirulina platensis ANS -1 were compared with reference CAS -10. The isolated strain ANS -1 and reference strain CAS 10 were grown in substrates 1/6 diluted Zarrouk’s medium (control), Rice mill effluent and sago industrial effluent.The effect of temperature and pH was determined for the strains CAS - 10 and ANS -1, and highest biomass was observed at 30°C of pH 9.0. In this optimum condition the biomass observed in CAS -10 and ANS - 1 was found to be 0.42 and 0.38 g 100ml respectively. The growth of Spirulina platensis was measured both in laboratory and outdoor condition by using the parameters viz., optical density, population, dry weight, protein and chlorophyll content. The high growth was recorded in 1/6 diluted Zarrouk’s medium followed by to rice mill effluent medium and sago industrial effluent. Maximum protein content was noticed in 1/6 diluted Zarrouk’s medium followed by rice mill effluent and sago industrial effluent.