Vinca rosea L. Syn. Catharanthus roseus L. G. Don. is an important medicinal plant which belongs to the family Apocynaceae. The present study deals with Micropropagation, Callogenesis, Cell suspension culture & Antimicrobial activity of Vinca rosea L.. Explants were cultured on MS basal medium and supplemented with different concentrations of BAP (mg/l) and NAA (mg/l) for micropropagation whereas 2, 4-D (mg/l) and Kin (mg/l) for callogenesis. Multiple shoots were obtained on all the concentrations of BAP and NAA, but BAP (1 mg/l) showed best response (90% & 80%).Similarly best callus response was observed on MS medium supplemented with 2, 4- D + Kin (1+1 mg/l) in all the explants (95% from leaf, 80% from node & 60% from fruit). Cell suspension culture have also been established in the present study according to which the calluses gave suspensions consisting of both the embryogenic and non-embryogenic cluster of the cells in 2, 4- D + Kin (1+1 mg/l) and gave best response (70%).The present study also describes the comparison of in vivo and in vitro antimicrobial activity of Vinca rosea. Three different pathogenic bacterial strains .) and three different fungal strains were used.