Over the years bovine mastitis is responsible for reduced milk production, leading to economic losses worldwide. In addition to being difficult to eradicate, mastitis requires antimicrobial therapy. In Brazil, despite the incidence of food poisoning caused by Staphylococcus aureus being unknown, previous studies indicate the important role of raw milk and dairy products in human outbreaks. Similarly, Staphylococcus spp. is the main genus of bacteria that causes mastitis in cattle. This book, therefore, provides the evaluation of the occurrence of biofilm-producing strains of Staphylococcus aureus in the milking environment of 10 farms located in state of São Paulo, Brazil. The analysis should help to improve mastitis control of the cattle by knowing the transmission route of the pathogen.