Charophyte research of 32 districts had been documented on extant charophytes (Characeae) from 1929-2010 within Bangladesh. In all, 41 taxa of charophytes were found,represented by five genera. These are Chara(19), Nitella(18) while Lychnothamnus,Lamprothamnium and Nitellopsis were reported by single taxa each. Two Nitella had been identified as new species in 2004,collected from rice field which were not yet described in home and abroad.These are: Nitella rajshahiensis Naz., Zaman., ex Diba sp. nov. and Nitella zamanii Naz ex Diba sp. nov. and first time these species had been included in this monograph.Major causes of charophytes threats were identified as:anthropogenic activity,modern mode of pisciculture,agricultural run-off all over Bangladesh and Farraka Barrage disaster is one of the major causes of habitat destruction for this group in the northern part of Bangladesh. All taxa are systematically described with their lucida drawings, photomicrograph illustrations, distribution and some ecological information.