This book contains an investigation carried out in twenty-eight durum wheat genotypes to find out the genetic diversity through morphological and molecular markers evaluated under randomized block design with three replications. The observation were recorded on ten quantitative traits viz., days to earhead emergence, grain filling period, days to maturity, plant height (cm), number of effective tillers per plant, spike length (cm), number of spikelets per spike, peduncle length, 1000 grain weight (g) and grain weight per plant (g).Different genetic parameters like genetic diversity, genetic advance, path coefficient analysis etc. were estimated. Twenty-eight genotypes were divided into seven clusters in genetic divergence study using SSR markers. The clustering pattern did not show any relationship between geographic distribution and genetic divergence as genotypes from the same origin scattered in different clusters and the genotypes of different areas were grouped in the same cluster.