Differentiation i.e. development of single cell into complex multicellular organs & tissues, results through selective gene expression which is specific to cell type, region & organ. Therefore, identification of biochemical markers during sequential stages of organogenesis could be used to identify early events of differentiation, embryogenic potential or serve as an indication of loss of regeneration capacity with culture age. Keeping in view these facts, this M. Phil. dissertation on T. indica, an important medicinal plant comprises of six chapters: Chapter-1 highlights background for selection of this plant species; Chapter-2 is compilation of relevant updated literature in the area of tissue culture, biochemical & SDS-PAGE studies; Chapter-3 deals with details of materials & techniques used; Chapter-4 presents the findings of study supported by tables, photographs & illustrations; Chapter-5 discusses the results in the light of available literature and Chapter-6 is summary of work followed by literature cited. This M. Phil. dissertation will be useful for reseachers working in the area of plant tissue culture & cell differentiation.