Untranslated regions (UTRs) are important regions in genes that are situated at 3? and 5? ends and are involved in the regulation of gene expression. UTRs on 3? end acts as complementary sequences for binding of microRNAs. The length and sequence of UTRs varies from species to species. In this work various conserved motifs in the untranslated regions of the genes have been reported by using software tool. Very little literature is available on the motif studies in the UTRs. This book therefore provides the insight on the presence of motifs with repeated single nucleotide in the 3? and 5? UTRs. Some coding genes were also analyzed for these motifs. These motifs show interesting similarities in different species of the genus Caenorhabditis. This finding could possibly be used in the identification of various organisms of same genus. These investigations could be helpful to scientific community working on non-coding RNAs, genomics and bioinformatics. This book could be helpful for students and teachers in the subject of Bioinformatics, Genomics, Molecular Biology and Biotechnology.