Endometrial carcinoma is one of the most common malignancies in the female genital tract and poses serious health hazards. The gene P53 safeguards cell integrity however; a mutation in this gene increases the lifeline of the cells which are programmed for cell death. This particular gene participates in many human cancers including endometrial, ovarian and tubal carcinomas. In this study we screened exon 5 of p53 gene to find out whether this exon is a hotspot for mutations or not. We evaluated (n=45) samples of endometrial carcinoma and (n=45) controls from a local population. However, we were unable to find any nucleotide alteration in exon 5. It appears, therefore, exon 5 of p53 is not a hot spot for mutation in local population based on recent our findings.