Myopia or nearsightedness is the most common reason for the development of impaired vision and visual disability. with a high prevalence around the globe, it has been investigated that among all the visual disabilities myopia is prevailing most rapidly with no discrimination of age and gender. Similarly, estimations have provided the evidence that about 4 billion people are affected from this disease in Pakistan. Myopic families from population of Pakistan were identified and were analyzed by microsatellite marker designed specifically for this disease. Genotypic analysis including Linkage analysis and LOD scores were calculated through bioinformatics softwares. It was revealed from the results that both clinical and genetic factors are involved in development of nearsightedness. This work has paved pathway for young scholars to understand molecular disease at a broader scale. Further study may identify any new locus responsible for causing the disease in this population. And this may lead to identification of novel gene. This will help in developing more advanced treatment techniques and suggesting new therapies for myopia treatment.