A combination of Nebivelol and Amlodipine is widely used in the treatment of cardiovascular diseases and hypertension. Hence it was decided to estimate simultaneously the presence of Nebivelol and Amlodipine in a formulation. A simple UV spectrophotometric methods were established and validated the methods as per IP and ICH guidelines and found to be applicable for routine analysis in an industry.Hence the combination of Nebivelol and Amlodipine can be evaluated with these methods, precisely in an industry. In the present work, a rapid, accurate and precise RP-HPLC method was developed for the simultaneous estimation of Nebivelol and Amlodipine in its Tablet dosage form [50mg each] by selecting the various chromatographic parameters. A new method was developed using gradient pump (LC-10AT vp pump). The method was validated by using various validation parameters like accuracy, precision, linearity. These results showed the method could find practical application as a quality control tool for analysis of the drug in its tablet dosage forms in quality control laboratories.