Cotton fibres contain naturally occurring non-cellulosic materials such as sugars, wax, metals and other organic species that may influence fibre characteristics, i.e. physical and chemical properties, yarn processing efficiency as well as product quality. Chemical and physical tests were conducted on raw cottons from different growing areas of Pakistan to determine the effect of different depositions of these materials on fibre properties, spinning performance and yarn properties. The present research study was planned to explore the location?s effect upon the non-cellulosic content of raw cotton and its ultimate effect on end product. In this concern four different growing areas (Multan, Bahawalpur, Rahim Yar Khan and Faisalabad), four cotton varieties CIM-473, CIM496, NIAB-111 and NIAB-999 with four micronaire values (4.4,4.6, 4.8 and5.0) were analyzed for wax, alcohol extractable, residual sugars, fibre ash contents and light metal content. Determined values were correlated to spinning consistency. Highly significant results were observed in respect of different locations, varieties and micronaire values.