Different kinds of viruses are the major causes of acute hepatitis worldwide. These viral infections are a main serious public health problem. The development of widely applicable techniques that are able to detect and quantify hepatitis viruses or the improvement of present methodology remains urgently needed. Detection of hepatitis viral infections has traditionally relied on the circulating antibody test using enzyme-linked immune-sorbent assay. However, a lot of novel tools are available recently and these technologies have been increasingly used to a variety of viral nucleic acid detections. They are proved to be superior to traditional methods in the considerable advantages of stability, rapidity, reproducibility as well as higher sensitivity and specificity. The present book is consisted by three original research articles. All of these manuscripts are based on experiments performed currently, mainly related to many novel strategies of hepatitis viruses nucleic acid amplification and quantification. The dedication of this work reflects the author’s anticipation that it would be helpful to some researchers and technicians in biomedical and viral diagnostic field.