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Opto-biochips for Microcytometry


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  • Product Description

Microscale fluorescence activated cell-sorting (micro-FACS) is a technology to identify and separate cells. Different elements of the micro-FACS, fabrication of opto-biochips, particle detection and velocity measurement, and flow switching, are studied in the present work. Biochips were fabricated by casting polydimethylsiloxane (PDMS) on the masters fabricated by direct laser-writing, and standard lithography and dry etching. In a different approach, biochips with integrated waveguides and microchannels were fabricated photolithographically on glass substrates using a negative photoresist, SU-8. In these opto-biochips, two excitation waveguides carrying two different wavelengths intersect the microchannel at two points separated and one large output waveguide carry fluorescence signals to a single filtered detector. The velocities of the microparticles were determined by the captured fluorescence from the double peak. Simulations based on beam propagation method were used to predict the performance of the SU-8 waveguides and the intensity at the two intersections has been compared with the experimental results.

Product Specifications
SKU :COC28121
AuthorSanket Goel
Number of Pages156
Publishing Year7/26/2010
Edition1 st
Book TypeElectronics & communications engineering
Country of Manufacture
Product BrandLAP LAMBERT Academic Publishing
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-07-29 00:00:00