The study showed the prevalence of Salmonella enteritidis and Salmonella typhimurium from poultry feed, meat and eggs and their role in the chain of transmission of salmonellae to human beings. The objective was to generate data to improve the quality of poultry products and human health awareness. Salmonellosis is one of the most wide spread food borne zoonoses. The etiological agents S. enteritidis and S. typhimurium not only produce the disease but during the convalescent phase remain carriers for indefinite period of time. In this study 400 samples were tested with Polymerase chain reaction(PCR) assay. There was no significant difference between layers feed and broilers feed as far as identification of salmonella enteritidis and salmonella typhimurium was concerned. It was found that 16%, 8%, 16% and 16% egg albumin was found positive for Salmonella enteritidis in layer egg albumin , Desi (local breed) egg albumin, double yolk albumin and broken egg albumin, respectively. The histo-pathology of different organs of broiler chickens at different phases of disease was also conducted for the better understanding of pathogenesis due to salmonellosis.