Maternal milk is an excellent source of nourishment for newborns as they get optimal balance of proteins, carbohydrates and fats from maternal milk. It provides a range of benefits for development, immunity, and growth. However, there is a possibility of contamination of maternal milk due to heavy use of pesticides in agriculture. To investigate the presence of any pesticide residues in the maternal milk; we have examined 135 milk samples from 45 mothers in Karachi, Pakistan. Among ten (10) pesticide residues investigated, only cypermethrin, permethrin, malathion, deltamethrin, and match were detected. Deltamethrin was present at the highest concentration followed by cypermethrin. These are alarming results and may have an undesired effect on newborn’s health; however, further research is required in vulnerable populations who are more exposed to this contamination. This information would be useful for farm workers, students of agriculture, and researchers in relevant fields.