Medicinal herbs are the local heritage with global importance.World is endowed with a rich wealth of medicinal herbs.The different variety of plants with different therapeutic properties is quiet astonishing. Herbs have provided us some of the very important life saving drugs. Among the estimated 4,00,000 plant species, only 6% have been studied for biological activity, and about 15% have been investigated phytochemically. This shows a need for investigation of herbal drugs and its phytochemical analysis.In present work,Jatropha gossypifolia Linn, it''s phytochemical analysis include study of medicinal uses, chemical constituents(specially terpenoids), morphological characters, physical evaluation, extractions using different solvents, phytochemical screening, separation and isolation by using TLC, column chromatography, HPTLC,quantitative estimation of active compound by HPTLC and UV, Structure elucidation of isolated compound by GCMS,1H NMR, IR, and finally pharmacological screening. Importance of Phytochemical analysis is modification of inactive natural products by suitable biological and chemical means into patent drug.