Xylocarpus moluccensis is an important medicinal plant having widespread application in a variety of disorders. The aim of this study was the phytochemical investigation and evaluation of the anti-oxidant, analgesic and anti-microbial activities of the bark of Xylocarpus moluccensis. Phytochemical screening of the ethanolic extract of Xylocarpus moluccensis ensured the presence of carbohydrate, reducing sugar, combined reducing sugar, glycosides, tannins, alkaloids, proteins, terpenoids and flavonoids. The anti-oxidant activity was measured by DPPH free radical scavenging activity (IC50 = ~17 µg/ml). The crude ethanolic extract exhibited dose dependent analgesic activity at a dose of 250 mg/kg and 500 mg/kg with 34.4% and 62.8 % inhibition of writhing respectively. In anti-microbial activity test by disc-diffusion method the extract showed activity against the bacterial strains namely S. aureus, S. epidermidis, E. coli, S. dysenteriae and proteus species at the dose of 250 µg/disc and 500µg/disc in comparison with standard drug Ciprofloxacin (5 µg/disc).