The present study investigates the anti-cancer and multi drug resistance (MDR) reversal potential of hydroalcoholic Allium cepa, Allium sativum, Boerhavia diffusa and Eclipta alba extracts through in vitro as well as in vivo experiments. Plant Extracts were able to induce apoptosis in both cell lines and cancer induced animals, promising result was given by EAE and ASE. RT-PCR analysis revealed that the mRNA expression of NF?B was markedly decreased in vitro and in vivo upon treated with the PE, preferably by EAE. Our data suggest the presence of bioactive compounds in PE, capable of augmenting liver carcinoma cells by lowering the level of MMP, ROS, induction of apoptosis, altered NF?B signaling and reversing MDR but among all extracts EAE shown the superlative result. These results suggest that EAE is a novel anti-cancer and potent MDR reversal agent and may be a potential adjunctive agent for tumor chemotherapy.