This study reveals the negative impact of diabetes mellitus on wound healing, both in experimental and clinical settings. Achyranthes aspera have been used in the traditional medicine for treatment of diabetes, wound healing, and many other complications. In the present investigation wound healing potential of ethanolic extract of Achyranthes aspera has been evaluated in normal treated and hyperglycemic rats. EEAA contains saponins, alkaloids, and flavonoids. Two weeks repeated dose treatment of EEAA, Reduced serum glucose level and restored body weight Produced antihyperglycemic effect. Reduced clotting time and haemoglobin content Showed faster wound contraction rate.Improved RBCs and WBCs count. Normalize the oxygen flow capacity and colour index. showed improvement in biochemical parameter estimation. Histopath study showed increased epithelisation of wound granulating tissue and protective effect on architecture of ? cells. SDS- PAGE analysis of wound tissue shows different proteins expression compared to normal animals. Thus it indicates that Achyranthes aspera possesses potential as a wound healing agent and also have antidiabetic activity.