This book has been aimed at Scientists, Research scholars, and Non specialized readers alike; this book retraces the basic practicals conducted in the laboratory for various biotechnology programs. The book describes the achievements and contains the important basic practical of Molecular Biology, Microbiology, Biochemistry, Genetic Engineering, Medical Biotechnology and Tissue culture (Plant & Animal) experiment running in various graduate and post graduate course of various international universities. The manual is written after all the practical were successfully obtained the result in the laboratory by the author. This book will give a detailed genesis practical of many areas of biotechnology especially Molecular Biology, Microbiology, Genetic Engineering and tissue culture. This book will help the reader to do the experiments with appropriate safety and best of the results. Every care has been taken to choose reliable sources of information.Any user of the book will do me a kindness if he reports to me any error that he may discover. If the new book should meet with the favor that fell to the old, I shall need these suggestions in the making of new edition also.