The current study was conducted to evaluate the oxidative stress status in prostate cancer. To achieve this purpose, 75 prostate cancer patients of ages 63.5± 3.5 years and 33 apparently healthy individuals of ages 61± 3.8 years (control group) were enrolled. The distribution of patients revealed 60% (40) of rural areas, 44% (30) are smokers, 52% (39) having either heart diseases and/or hypertension, and 40% (30) of positive family history for the disease. The levels of MDA, GSH, GPX, and CAT were measured in sera of patients and control group. The statistical analysis exhibited significant elevation for MDA levels and significant decreases for GSH and GPX levels in the group of patients when compared with those of the control group. Patients from rural areas showed significant rise for MDA values when compared with those of urban areas. Variations of oxidative stress markers were found to be essentially related to prostate cancer and independent on smoking or other diseases in the studied patients. MDA levels were demonstrated to increase significantly in patients of positive family history when compared with those of negative family history of prostate cancer.