Although the prevalence of dental caries in children has declined markedly over the last two decades in most countries, the disease continues to be a major problem for both children and adults everywhere. In recent year’s management of dental caries moved toward an antibiotic/antimicrobial model of disease prevention. This approach, however, raises serious questions: Firstly, do the antibiotic/antimicrobial agents (chlorhexidine, povidone-iodine, fluoride, etc.) kill all offending organisms? Secondly, if so, do the agents preclude the re-entry of the same organisms from external sources? And finally, if the agents do kill all the offending organisms , do any remaining pathogenic organisms have selective advantage in repopulating the tooth surfaces? To overcome the problems inherent in an antibiotic/antimicrobial approach, recently in dentistry bacterial interference with Probiotics to support the stability and diversity of oral biofilms has gained interest.