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Production of Recombinant RANKL for in vitro Osteoclastogenesis


Marketed By :  AV Akademikerverlag   Sold By :  Kamal Books International  
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Rs. 2,675

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  • Product Description

Receptor Activator of Nuclear Factor κ B Ligand (RANKL) is a protein that belongs to the family of tumour necrosis factors (TNF) and is one of the two main inducers of osteoclast differentiation. It is therefore used in research facilities that study the molecular and cellular backgrounds of osteoarthritis (OA), a degenerative bone disease that is thought to be associated with a decline in osteoclast number or function. The aim of this work was to produce RANKL, using classical as well as novel methods and techniques of molecular biology and cell culture, including screening for the gene RANKL in leukaemic cell lines, amplification, transformation of Escherichia coli (E. coli) with a plasmid in which RANKL was previously inserted and subsequent analysis of clones, transfection of Chinese Hamster Ovary (CHO) cells with the purified plasmid, and finally qualitative analysis of the protein RANKL in the supernatant of a transfected CHO culture.

Product Specifications
SKU :COC56101
AuthorSilke Machauer
Number of Pages64
Publishing Year2014-06-26T00:00:00.000
Edition1 st
Book TypeBiology, life sciences
Country of ManufactureIndia
Product BrandAV Akademikerverlag
Product Packaging InfoBox
In The Box1 Piece
Product First Available On ClickOnCare.com2015-06-08 00:00:00