Finding effective biocontrol PGPR strains for fundamental research or practical applications requires a combination of ingenuity and hard work. Attempts to use fluorescent Pseudomonas to improve crop yield have enormous potential, but to date they have only limited success which is in part due to the lack of understanding of their population dynamics in environment and the bacterial traits involved in the process of rhizosphere competence. The potential and enormous impact of soil bacteria such as fluorescent Pseudomonas can have on plants prompted to take the present investigation “Exploiting Genotypic Diversity & Plant Growth Promoting Activity (PGPA) of Pseudomonas fluorescens”. The genotypic diversity of antibiotic-producing Pseudomonas spp. provides an enormous resource for identifying strains that are highly rhizosphere competent and superior for biological control of plant diseases.